1 00:00:02,869 --> 00:00:01,429 hi i'm devin payne and i'm going to be 2 00:00:04,230 --> 00:00:02,879 talking about the reductive dissolution 3 00:00:05,910 --> 00:00:04,240 of pyrite by methanogens and its 4 00:00:07,349 --> 00:00:05,920 physiological consequences 5 00:00:09,430 --> 00:00:07,359 so we've shown that methanogens can 6 00:00:10,790 --> 00:00:09,440 reduce pyrite by growing these cells 7 00:00:12,070 --> 00:00:10,800 with either pyrite or chronological 8 00:00:13,509 --> 00:00:12,080 forms of iron and sulfur 9 00:00:14,950 --> 00:00:13,519 and we see that when the cells grow with 10 00:00:16,630 --> 00:00:14,960 pyrite they reduce it and produce 11 00:00:17,910 --> 00:00:16,640 sulfide that's measurable in solution in 12 00:00:20,310 --> 00:00:17,920 our biotic cultures 13 00:00:21,750 --> 00:00:20,320 but not in our abiotic microcosms and 14 00:00:23,189 --> 00:00:21,760 this research was published in the isme 15 00:00:24,790 --> 00:00:23,199 journal this year where we also showed 16 00:00:26,230 --> 00:00:24,800 that methanogens require direct surface 17 00:00:27,349 --> 00:00:26,240 access to pyrite to grow 18 00:00:28,790 --> 00:00:27,359 where when cells are provided with 19 00:00:29,509 --> 00:00:28,800 pyrite that's green solution they grow 20 00:00:31,109 --> 00:00:29,519 very well 21 00:00:32,709 --> 00:00:31,119 however when pirates sequester dialysis 22 00:00:34,310 --> 00:00:32,719 tubing we see no growth and this 23 00:00:36,470 --> 00:00:34,320 finding was further corroborated by our 24 00:00:38,630 --> 00:00:36,480 observations of cells growing in close 25 00:00:40,470 --> 00:00:38,640 contact with the minerals 26 00:00:42,630 --> 00:00:40,480 this growth on pirate has physiological 27 00:00:43,990 --> 00:00:42,640 effects at the level of cell size and 28 00:00:45,750 --> 00:00:44,000 the amount of iron per cell 29 00:00:47,510 --> 00:00:45,760 where pyrite grown cells are smaller and 30 00:00:49,350 --> 00:00:47,520 accumulate more iron than ferrous iron 31 00:00:51,110 --> 00:00:49,360 and sulfide grown cells 32 00:00:53,029 --> 00:00:51,120 this research was recently accepted in 33 00:00:55,029 --> 00:00:53,039 the journal bacteriology 34 00:00:57,430 --> 00:00:55,039 with that i would like to acknowledge my 35 00:00:59,110 --> 00:00:57,440 mentor dr eric spoyd our boyd lab 36 00:01:01,110 --> 00:00:59,120 members collaborators and our funding 37 00:01:01,590 --> 00:01:01,120 sources which includes the department of